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細胞培養プロファイリング

概要

 培養上清の多成分一斉分析により培養細胞の詳細な培養プロファイルを測定するプラットフォームにより、ビタミンなどの微量培地成分とグルコースやアミノ酸を単一のシステムで測定します。
 島津製作所の「細胞培養プロファイリングメソッドパッケージ」(Ver.2)を用いて、培地成分および細胞からの分泌代謝物から成る合計125成分を一斉分析可能なメソッドです。旧バージョンから、アミノ酸代謝、核酸代謝、TCAサイクルに関わる代謝物などを測定対象に追加することで、より詳細な細胞培養プロファイルの情報取得が可能です。

分析・試験項目

Amino acids and their
metabolites
Group
5-Oxoproline Ala, Asp and Glu MT
Alanine Ala, Asp and Glu MT
Asparagine Ala, Asp and Glu MT
Aspartic acid Ala, Asp and Glu MT
Glutamic acid Ala, Asp and Glu MT
Glutamine Ala, Asp and Glu MT
N-Acetylaspartic acid Ala, Asp and Glu MT
4-Aminobutyric acid Arg MT
4-Hydroxyproline Arg MT
Arginine Arg MT
Citrulline Arg MT
Ornithine Arg MT
Proline Arg MT
Putrescine Arg MT
Argininosuccinic acid Arg MT
2-Aminobutyric acid Cys and Met MT
5-Glutamylcysteine Cys and Met MT
5'-Methylthioadenosine Cys and Met MT
Cystathionine Cys and Met MT
Cysteine Cys and Met MT
Cystine Cys and Met MT
Glutathione Cys and Met MT
Homocysteine Cys and Met MT
Methionine Cys and Met MT
Methionine sulfoxide Cys and Met MT
N-Acetylcysteine Cys and Met MT
Oxidized glutathione Cys and Met MT
S-Adenosylhomocysteine Cys and Met MT
Glycine Gly and Ser MT
Serine Gly and Ser MT
Threonine Gly and Ser MT
1-Methylhistidine His MT
3-Methylhistidine His MT
Histidine His MT
Urocanic acid His MT
2-Aminoadipic acid Lys MT
Hydroxylysine Lys MT
Lysine Lys MT
Pipecolic acid Lys MT
Saccharopine Lys MT
3-Hydroxyanthranilic acid Trp MT
5-Hydroxytryptophan Trp MT
Anthranilic acid Trp MT
Formylkynurenine Trp MT
Hydroxykynurenine Trp MT
Indole-3-acetic acid Trp MT
Kynurenic acid Trp MT
Kynurenine Trp MT
Serotonin Trp MT
Tryptophan Trp MT
4-Hydroxyphenyllactic acid Tyr MT
Phenylalanine Tyr MT
Tyrosine Tyr MT
3-Hydroxyisobutyric acid Val MT
3-Methyl-2-oxovaleric acid Val MT
Isoleucine Val MT
Leucine Val MT
Valine Val MT
Alanyl-glutamine
Glycyl-glutamine
Others Group
2-ketoglutaric acid TCA Cycle
Acotinic acid TCA Cycle
Citric acid TCA Cycle
Fumaric acid TCA Cycle
Isocitric acid TCA Cycle
Lactic acid TCA Cycle
Malic acid TCA Cycle
Pyruvic acid TCA Cycle
Succinic acid TCA Cycle
Penicillin G Antibiotics
2-Aminoethanol
Glyceric acid
NAD
O-Phosphoethanolamine
Taurine
対象化合物一覧
Nucleic acids and their
metabolites
Group
Adenine Purine MT
Adenosine Purine MT
Adenosine monophosphate Purine MT
Deoxyadenosine Purine MT
Deoxyadenosine monophosphate Purine MT
Deoxyguanosine Purine MT
Deoxyguanosine monophosphate Purine MT
Guanine Purine MT
Guanosine Purine MT
Guanosine monophosphate Purine MT
Hypoxanthine Purine MT
Inosine Purine MT
Inosine monophosphate Purine MT
Uric acid Purine MT
Xanthine Purine MT
Xanthosine Purine MT
Xanthosine monophosphate Purine MT
3-Aminoisobutyric acid Pyrimidine MT
3-Aminopropanoic acid Pyrimidine MT
Cytidine Pyrimidine MT
Cytidine monophosphate Pyrimidine MT
Cytosine Pyrimidine MT
Deoxycytidine Pyrimidine MT
Deoxycytidine monophosphate Pyrimidine MT
Orotic acid Pyrimidine MT
Thymidine Pyrimidine MT
Thymidine monophosphate Pyrimidine MT
Thymine Pyrimidine MT
Uracil Pyrimidine MT
Uridine Pyrimidine MT
Uridine monophosphate Pyrimidine MT
Sugars Group
Gluconic acid
Hexose (Glucose)
Sucrose
Threonic acid
Vitamins Group
Riboflavin B2
Niacinamide B3
Nicotinic acid B3
Pantothenic acid B5
4-Pyridoxic acid B6
Pyridoxal B6
Pyridoxalphosphate B6
Pyridoxine B6
Biotin B7
4-Aminobenzoic acid B9
Folic acid B9
Choline B12
Ascorbic acid C
Cyanocobalamin Vitamin-like
Lipoic acid Vitamin-like
Internal standard Group
2-Isopropylmalic acid

 対象化合物一覧は、島津製作所WEBサイトより引用 

Amino acids and their
metabolites
Group
5-Oxoproline Ala, Asp and Glu MT
Alanine Ala, Asp and Glu MT
Asparagine Ala, Asp and Glu MT
Aspartic acid Ala, Asp and Glu MT
Glutamic acid Ala, Asp and Glu MT
Glutamine Ala, Asp and Glu MT
N-Acetylaspartic acid Ala, Asp and Glu MT
4-Aminobutyric acid Arg MT
4-Hydroxyproline Arg MT
Arginine Arg MT
Citrulline Arg MT
Ornithine Arg MT
Proline Arg MT
Putrescine Arg MT
Argininosuccinic acid Arg MT
2-Aminobutyric acid Cys and Met MT
5-Glutamylcysteine Cys and Met MT
5'-Methylthioadenosine Cys and Met MT
Cystathionine Cys and Met MT
Cysteine Cys and Met MT
Cystine Cys and Met MT
Glutathione Cys and Met MT
Homocysteine Cys and Met MT
Methionine Cys and Met MT
Methionine sulfoxide Cys and Met MT
N-Acetylcysteine Cys and Met MT
Oxidized glutathione Cys and Met MT
S-Adenosylhomocysteine Cys and Met MT
Glycine Gly and Ser MT
Serine Gly and Ser MT
Threonine Gly and Ser MT
1-Methylhistidine His MT
3-Methylhistidine His MT
Histidine His MT
Urocanic acid His MT
2-Aminoadipic acid Lys MT
Hydroxylysine Lys MT
Lysine Lys MT
Pipecolic acid Lys MT
Saccharopine Lys MT
3-Hydroxyanthranilic acid Trp MT
5-Hydroxytryptophan Trp MT
Anthranilic acid Trp MT
Formylkynurenine Trp MT
Hydroxykynurenine Trp MT
Indole-3-acetic acid Trp MT
Kynurenic acid Trp MT
Kynurenine Trp MT
Serotonin Trp MT
Tryptophan Trp MT
4-Hydroxyphenyllactic acid Tyr MT
Phenylalanine Tyr MT
Tyrosine Tyr MT
3-Hydroxyisobutyric acid Val MT
3-Methyl-2-oxovaleric acid Val MT
Isoleucine Val MT
Leucine Val MT
Valine Val MT
Alanyl-glutamine
Glycyl-glutamine
Others Group
2-ketoglutaric acid TCA Cycle
Acotinic acid TCA Cycle
Citric acid TCA Cycle
Fumaric acid TCA Cycle
Isocitric acid TCA Cycle
Lactic acid TCA Cycle
Malic acid TCA Cycle
Pyruvic acid TCA Cycle
Succinic acid TCA Cycle
Penicillin G Antibiotics
2-Aminoethanol
Glyceric acid
NAD
O-Phosphoethanolamine
Taurine
対象化合物一覧
Nucleic acids and their
metabolites
Group
Adenine Purine MT
Adenosine Purine MT
Adenosine monophosphate Purine MT
Deoxyadenosine Purine MT
Deoxyadenosine monophosphate Purine MT
Deoxyguanosine Purine MT
Deoxyguanosine monophosphate Purine MT
Guanine Purine MT
Guanosine Purine MT
Guanosine monophosphate Purine MT
Hypoxanthine Purine MT
Inosine Purine MT
Inosine monophosphate Purine MT
Uric acid Purine MT
Xanthine Purine MT
Xanthosine Purine MT
Xanthosine monophosphate Purine MT
3-Aminoisobutyric acid Pyrimidine MT
3-Aminopropanoic acid Pyrimidine MT
Cytidine Pyrimidine MT
Cytidine monophosphate Pyrimidine MT
Cytosine Pyrimidine MT
Deoxycytidine Pyrimidine MT
Deoxycytidine monophosphate Pyrimidine MT
Orotic acid Pyrimidine MT
Thymidine Pyrimidine MT
Thymidine monophosphate Pyrimidine MT
Thymine Pyrimidine MT
Uracil Pyrimidine MT
Uridine Pyrimidine MT
Uridine monophosphate Pyrimidine MT
Sugars Group
Gluconic acid
Hexose (Glucose)
Sucrose
Threonic acid
Vitamins Group
Riboflavin B2
Niacinamide B3
Nicotinic acid B3
Pantothenic acid B5
4-Pyridoxic acid B6
Pyridoxal B6
Pyridoxalphosphate B6
Pyridoxine B6
Biotin B7
4-Aminobenzoic acid B9
Folic acid B9
Choline B12
Ascorbic acid C
Cyanocobalamin Vitamin-like
Lipoic acid Vitamin-like
Internal standard Group
2-Isopropylmalic acid

 対象化合物一覧は、島津製作所WEBサイトより引用 

関連情報

20201001